A29 Wolbachia for dengue control; will dengue viruses evolve resistance?

represents the dominant virus subtype. It does not provide sufficient information to resolve transmission events particularly for rapidly spreading viruses. However, changes in the composition of minor variants between hosts and the pattern of minor variants fixation during outbreaks, could provide additional high-resolution data on who is infecting whom. The same data could also potentially inform the extent of within-host virus diversity as well as the proportion of diversity that is transmitted between individuals. We have developed a reproducible semi-automated whole genome variant calling pipeline to explore the role of minority variants in resolving transmission patterns and within host viral evolution. The pipeline is available as modular Bash scripts that run on a Linux cluster environment.